Journal: Horticulture Research

Article Title: Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality

doi: 10.1038/s41438-021-00611-1

Figure Lengend Snippet: All constructs were infiltrated at OD 600 = 0.8, and images were taken 5 days after infiltration. a – d Representative images of fluorescent protein fusions localized to the Golgi apparatus (ST-GFP), ER (RFP-HDEL), actin cytoskeleton (GFP-Lifeact), and plastids (PT-RFP). e Transient expression of mCherry-HY5 (a transcription factor) localized to the nucleus that was colabeled with H2B-GFP (a nuclear marker). f Coexpression of GFP-HDEL and ST-RFP identified the close association between the ER network and Golgi bodies in kumquat fruit cells. Scale bar, 10 μm

Article Snippet: A total amount of 30 μg protein was used for western blot analysis and detected by either GFP or RFP antibodies (Biorbyt) at a dilution of 1:5000 or 1:10,000, respectively.

Techniques: Construct, Expressing, Marker